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Stimulation Device, supplied by Micromed Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stimulation device  (Aurora Scientific)
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Active force generation is reduced in 5 d.p.f. uts2r3 mutant larval trunks. (A) Schematic of the ex vivo larval trunk mechanics assay. Isolated 5 d.p.f. preparations were mounted between a length motor and force transducer for mechanical testing. Sarcomere striations were visualized and used to measure muscle length before mechanical assessment. (A’) Representative image of a mounted 5 d.p.f. trunk preparation, with the expanded view showing sarcomere striations. (B) Cross-sectional area of larval trunk preparations, calculated from dorsal-ventral and lateral diameter measurements assuming an elliptical cross section. Cross-sectional area did not differ between wild-type and uts2r3 mutant specimens ( p = 0.495; WT, n = 10; uts2r3 , n = 11). (C) Maximal tension generated during twitch and tetanic <t>stimulation.</t> uts2r3 mutants generated reduced tension compared with wild type, with a significant reduction during tetanic stimulation. WT, n = 9; uts2r3 , n = 10. (D) Maximal force generated during twitch and tetanic stimulation. uts2r3 mutants generated significantly less force under both stimulation conditions. WT, n = 9; uts2r3 , n = 10. (E) Force depression during shortening contractions, expressed as percent reduction from isometric force at the same length. No significant differences were detected across contractile velocities. WT, n = 8; uts2r3 , n = 7. Bars show means ± SD with individual specimens shown as points.
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portable stimulation device  (Chattanooga Group)
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Active force generation is reduced in 5 d.p.f. uts2r3 mutant larval trunks. (A) Schematic of the ex vivo larval trunk mechanics assay. Isolated 5 d.p.f. preparations were mounted between a length motor and force transducer for mechanical testing. Sarcomere striations were visualized and used to measure muscle length before mechanical assessment. (A’) Representative image of a mounted 5 d.p.f. trunk preparation, with the expanded view showing sarcomere striations. (B) Cross-sectional area of larval trunk preparations, calculated from dorsal-ventral and lateral diameter measurements assuming an elliptical cross section. Cross-sectional area did not differ between wild-type and uts2r3 mutant specimens ( p = 0.495; WT, n = 10; uts2r3 , n = 11). (C) Maximal tension generated during twitch and tetanic <t>stimulation.</t> uts2r3 mutants generated reduced tension compared with wild type, with a significant reduction during tetanic stimulation. WT, n = 9; uts2r3 , n = 10. (D) Maximal force generated during twitch and tetanic stimulation. uts2r3 mutants generated significantly less force under both stimulation conditions. WT, n = 9; uts2r3 , n = 10. (E) Force depression during shortening contractions, expressed as percent reduction from isometric force at the same length. No significant differences were detected across contractile velocities. WT, n = 8; uts2r3 , n = 7. Bars show means ± SD with individual specimens shown as points.
Portable Stimulation Device, supplied by Chattanooga Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Active force generation is reduced in 5 d.p.f. uts2r3 mutant larval trunks. (A) Schematic of the ex vivo larval trunk mechanics assay. Isolated 5 d.p.f. preparations were mounted between a length motor and force transducer for mechanical testing. Sarcomere striations were visualized and used to measure muscle length before mechanical assessment. (A’) Representative image of a mounted 5 d.p.f. trunk preparation, with the expanded view showing sarcomere striations. (B) Cross-sectional area of larval trunk preparations, calculated from dorsal-ventral and lateral diameter measurements assuming an elliptical cross section. Cross-sectional area did not differ between wild-type and uts2r3 mutant specimens ( p = 0.495; WT, n = 10; uts2r3 , n = 11). (C) Maximal tension generated during twitch and tetanic <t>stimulation.</t> uts2r3 mutants generated reduced tension compared with wild type, with a significant reduction during tetanic stimulation. WT, n = 9; uts2r3 , n = 10. (D) Maximal force generated during twitch and tetanic stimulation. uts2r3 mutants generated significantly less force under both stimulation conditions. WT, n = 9; uts2r3 , n = 10. (E) Force depression during shortening contractions, expressed as percent reduction from isometric force at the same length. No significant differences were detected across contractile velocities. WT, n = 8; uts2r3 , n = 7. Bars show means ± SD with individual specimens shown as points.
1 × 1 Tdcs Mini Ct Stimulator Device, supplied by Soterix Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Active force generation is reduced in 5 d.p.f. uts2r3 mutant larval trunks. (A) Schematic of the ex vivo larval trunk mechanics assay. Isolated 5 d.p.f. preparations were mounted between a length motor and force transducer for mechanical testing. Sarcomere striations were visualized and used to measure muscle length before mechanical assessment. (A’) Representative image of a mounted 5 d.p.f. trunk preparation, with the expanded view showing sarcomere striations. (B) Cross-sectional area of larval trunk preparations, calculated from dorsal-ventral and lateral diameter measurements assuming an elliptical cross section. Cross-sectional area did not differ between wild-type and uts2r3 mutant specimens ( p = 0.495; WT, n = 10; uts2r3 , n = 11). (C) Maximal tension generated during twitch and tetanic <t>stimulation.</t> uts2r3 mutants generated reduced tension compared with wild type, with a significant reduction during tetanic stimulation. WT, n = 9; uts2r3 , n = 10. (D) Maximal force generated during twitch and tetanic stimulation. uts2r3 mutants generated significantly less force under both stimulation conditions. WT, n = 9; uts2r3 , n = 10. (E) Force depression during shortening contractions, expressed as percent reduction from isometric force at the same length. No significant differences were detected across contractile velocities. WT, n = 8; uts2r3 , n = 7. Bars show means ± SD with individual specimens shown as points.
Electro Stimulation Device Ed, supplied by Autodesk Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Active force generation is reduced in 5 d.p.f. uts2r3 mutant larval trunks. (A) Schematic of the ex vivo larval trunk mechanics assay. Isolated 5 d.p.f. preparations were mounted between a length motor and force transducer for mechanical testing. Sarcomere striations were visualized and used to measure muscle length before mechanical assessment. (A’) Representative image of a mounted 5 d.p.f. trunk preparation, with the expanded view showing sarcomere striations. (B) Cross-sectional area of larval trunk preparations, calculated from dorsal-ventral and lateral diameter measurements assuming an elliptical cross section. Cross-sectional area did not differ between wild-type and uts2r3 mutant specimens ( p = 0.495; WT, n = 10; uts2r3 , n = 11). (C) Maximal tension generated during twitch and tetanic <t>stimulation.</t> uts2r3 mutants generated reduced tension compared with wild type, with a significant reduction during tetanic stimulation. WT, n = 9; uts2r3 , n = 10. (D) Maximal force generated during twitch and tetanic stimulation. uts2r3 mutants generated significantly less force under both stimulation conditions. WT, n = 9; uts2r3 , n = 10. (E) Force depression during shortening contractions, expressed as percent reduction from isometric force at the same length. No significant differences were detected across contractile velocities. WT, n = 8; uts2r3 , n = 7. Bars show means ± SD with individual specimens shown as points.
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Active force generation is reduced in 5 d.p.f. uts2r3 mutant larval trunks. (A) Schematic of the ex vivo larval trunk mechanics assay. Isolated 5 d.p.f. preparations were mounted between a length motor and force transducer for mechanical testing. Sarcomere striations were visualized and used to measure muscle length before mechanical assessment. (A’) Representative image of a mounted 5 d.p.f. trunk preparation, with the expanded view showing sarcomere striations. (B) Cross-sectional area of larval trunk preparations, calculated from dorsal-ventral and lateral diameter measurements assuming an elliptical cross section. Cross-sectional area did not differ between wild-type and uts2r3 mutant specimens ( p = 0.495; WT, n = 10; uts2r3 , n = 11). (C) Maximal tension generated during twitch and tetanic <t>stimulation.</t> uts2r3 mutants generated reduced tension compared with wild type, with a significant reduction during tetanic stimulation. WT, n = 9; uts2r3 , n = 10. (D) Maximal force generated during twitch and tetanic stimulation. uts2r3 mutants generated significantly less force under both stimulation conditions. WT, n = 9; uts2r3 , n = 10. (E) Force depression during shortening contractions, expressed as percent reduction from isometric force at the same length. No significant differences were detected across contractile velocities. WT, n = 8; uts2r3 , n = 7. Bars show means ± SD with individual specimens shown as points.
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animal dcs stimulator model 2100 device  (Soterix Medical)
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Active force generation is reduced in 5 d.p.f. uts2r3 mutant larval trunks. (A) Schematic of the ex vivo larval trunk mechanics assay. Isolated 5 d.p.f. preparations were mounted between a length motor and force transducer for mechanical testing. Sarcomere striations were visualized and used to measure muscle length before mechanical assessment. (A’) Representative image of a mounted 5 d.p.f. trunk preparation, with the expanded view showing sarcomere striations. (B) Cross-sectional area of larval trunk preparations, calculated from dorsal-ventral and lateral diameter measurements assuming an elliptical cross section. Cross-sectional area did not differ between wild-type and uts2r3 mutant specimens ( p = 0.495; WT, n = 10; uts2r3 , n = 11). (C) Maximal tension generated during twitch and tetanic <t>stimulation.</t> uts2r3 mutants generated reduced tension compared with wild type, with a significant reduction during tetanic stimulation. WT, n = 9; uts2r3 , n = 10. (D) Maximal force generated during twitch and tetanic stimulation. uts2r3 mutants generated significantly less force under both stimulation conditions. WT, n = 9; uts2r3 , n = 10. (E) Force depression during shortening contractions, expressed as percent reduction from isometric force at the same length. No significant differences were detected across contractile velocities. WT, n = 8; uts2r3 , n = 7. Bars show means ± SD with individual specimens shown as points.
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ti stimulation device  (Soterix Medical)
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Active force generation is reduced in 5 d.p.f. uts2r3 mutant larval trunks. (A) Schematic of the ex vivo larval trunk mechanics assay. Isolated 5 d.p.f. preparations were mounted between a length motor and force transducer for mechanical testing. Sarcomere striations were visualized and used to measure muscle length before mechanical assessment. (A’) Representative image of a mounted 5 d.p.f. trunk preparation, with the expanded view showing sarcomere striations. (B) Cross-sectional area of larval trunk preparations, calculated from dorsal-ventral and lateral diameter measurements assuming an elliptical cross section. Cross-sectional area did not differ between wild-type and uts2r3 mutant specimens ( p = 0.495; WT, n = 10; uts2r3 , n = 11). (C) Maximal tension generated during twitch and tetanic <t>stimulation.</t> uts2r3 mutants generated reduced tension compared with wild type, with a significant reduction during tetanic stimulation. WT, n = 9; uts2r3 , n = 10. (D) Maximal force generated during twitch and tetanic stimulation. uts2r3 mutants generated significantly less force under both stimulation conditions. WT, n = 9; uts2r3 , n = 10. (E) Force depression during shortening contractions, expressed as percent reduction from isometric force at the same length. No significant differences were detected across contractile velocities. WT, n = 8; uts2r3 , n = 7. Bars show means ± SD with individual specimens shown as points.
Ti Stimulation Device, supplied by Soterix Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Active force generation is reduced in 5 d.p.f. uts2r3 mutant larval trunks. (A) Schematic of the ex vivo larval trunk mechanics assay. Isolated 5 d.p.f. preparations were mounted between a length motor and force transducer for mechanical testing. Sarcomere striations were visualized and used to measure muscle length before mechanical assessment. (A’) Representative image of a mounted 5 d.p.f. trunk preparation, with the expanded view showing sarcomere striations. (B) Cross-sectional area of larval trunk preparations, calculated from dorsal-ventral and lateral diameter measurements assuming an elliptical cross section. Cross-sectional area did not differ between wild-type and uts2r3 mutant specimens ( p = 0.495; WT, n = 10; uts2r3 , n = 11). (C) Maximal tension generated during twitch and tetanic stimulation. uts2r3 mutants generated reduced tension compared with wild type, with a significant reduction during tetanic stimulation. WT, n = 9; uts2r3 , n = 10. (D) Maximal force generated during twitch and tetanic stimulation. uts2r3 mutants generated significantly less force under both stimulation conditions. WT, n = 9; uts2r3 , n = 10. (E) Force depression during shortening contractions, expressed as percent reduction from isometric force at the same length. No significant differences were detected across contractile velocities. WT, n = 8; uts2r3 , n = 7. Bars show means ± SD with individual specimens shown as points.

Journal: bioRxiv

Article Title: Active and Passive Mechanical Deficits Precede Spinal Curvature in a Zebrafish Model of Idiopathic Scoliosis

doi: 10.64898/2026.04.29.721663

Figure Lengend Snippet: Active force generation is reduced in 5 d.p.f. uts2r3 mutant larval trunks. (A) Schematic of the ex vivo larval trunk mechanics assay. Isolated 5 d.p.f. preparations were mounted between a length motor and force transducer for mechanical testing. Sarcomere striations were visualized and used to measure muscle length before mechanical assessment. (A’) Representative image of a mounted 5 d.p.f. trunk preparation, with the expanded view showing sarcomere striations. (B) Cross-sectional area of larval trunk preparations, calculated from dorsal-ventral and lateral diameter measurements assuming an elliptical cross section. Cross-sectional area did not differ between wild-type and uts2r3 mutant specimens ( p = 0.495; WT, n = 10; uts2r3 , n = 11). (C) Maximal tension generated during twitch and tetanic stimulation. uts2r3 mutants generated reduced tension compared with wild type, with a significant reduction during tetanic stimulation. WT, n = 9; uts2r3 , n = 10. (D) Maximal force generated during twitch and tetanic stimulation. uts2r3 mutants generated significantly less force under both stimulation conditions. WT, n = 9; uts2r3 , n = 10. (E) Force depression during shortening contractions, expressed as percent reduction from isometric force at the same length. No significant differences were detected across contractile velocities. WT, n = 8; uts2r3 , n = 7. Bars show means ± SD with individual specimens shown as points.

Article Snippet: Stimulation was provided using a constant-current stimulation device (Aurora Scientific), with supramaximal current determined independently for each sample preparation before mechanical assessment.

Techniques: Mutagenesis, Ex Vivo, Isolation, Generated